Fig. 3. Effects of ANG concentration (A, B), washing (C) and serum (D, FBS) on ANG fluorescence in the P1 cell subset. The displayed histograms were obtained in independent experiments with identical instrument settings. (B) Dependence of cell ANG fluorescence on ANG-AM concentration in serum-free RPMI media, for 2 experiments, as shown in Fig. 3A.